Integrin alpha M (ITGAM) antibody

Antigen: Integrin alpha M (ITGAM)

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Clonality: Monoclonal (M1-70)

Host: Rat

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN155493

Additional Information

Alternative name: CD11b

Immunogen: C57BL/10 splenocytes

Format: Purified

Isotype: IgG2b, kappa chain  (Matching secondary antibodies)

Clone: M1-70

Description: CD11b is a 170 kD glycoprotein, also known as alphaM integrin, Mac-1 alpha subunit, Mol, CR3, or Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, NK cells, and some T and B cells. CD11b non-covalently associates with CD18 (beta2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. The M1/70 antibody can block CD11b-mediated cellular adhesion. This antibody is also cross-reactive with human CD11b. Distribution: Granulocytes, monocytes, NK cells, some T and B cells Function: Adhesion, chemotaxis Synonyms: alphaM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR Structure: Integrin family, associates with integrin ß2 (CD18), 170 kD

Application Details

Application Notes: Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation, in vitro blocking of cell-cell adhesion, and immunohistochemistry of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155505). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 °C.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

BALB/c mouse bone marrow cells stained with purified M1/70, followed by anti-rat IgGs FITC

Publications

Publications: Springer, Galfre, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Springer, Davignon, Ho et al.: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, 1983 (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Coxon, Rieu, Barkalow et al.: "A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation." in: Immunity, Vol. 5, Issue 6, pp. 653-66, 1997 (PubMed).

Zhang, McCormick, Desai et al.: "Murine sclerodermatous graft-versus-host disease, a model for human scleroderma: cutaneous cytokines, chemokines, and immune cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 6, pp. 3088-98, 2002 (PubMed).

Hata, Sakaguchi, Yoshitomi et al.: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004 (PubMed).

Olver, Groves, Buttigieg et al.: "Tumor-derived interleukin-4 reduces tumor clearance and deviates the cytokine and granzyme profile of tumor-induced CD8+ T cells." in: Cancer research, Vol. 66, Issue 1, pp. 571-80, 2006 (PubMed).

Ponomarev, Shriver, Dittel: "CD40 expression by microglial cells is required for their completion of a two-step activation process during central nervous system autoimmune inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1402-10, 2006 (PubMed).

Tan, Zhao, Bi et al.: "Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2872-9, 2006 (PubMed).

Dzhagalov, St John, He: "The antiapoptotic protein Mcl-1 is essential for the survival of neutrophils but not macrophages." in: Blood, Vol. 109, Issue 4, pp. 1620-6, 2007 (PubMed).

Fazilleau, Eisenbraun, Malherbe et al.: "Lymphoid reservoirs of antigen-specific memory T helper cells." in: Nature immunology, Vol. 8, Issue 7, pp. 753-61, 2007 (PubMed).