CD18 antibody (PE)

Antigen: CD18

Clonality: Monoclonal (M18-2)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN155538

Additional Information

Immunogen: Mouse CTL glycoprotein

Isotype: IgG2a, kappa chain

Clone: M18-2

Description: CD18 is a 95 kD protein, also known as integrin beta2 subunit. It is expressed on all leukocytes. CD18, in association with integrin alpha chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and alphaXbeta2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion. Distribution: All leukocytes Function: Adhesion Synonyms: integrin beta2, beta2 integrin Structure: Integrin family, associates with CD11a, CD11b, CD11c, 95 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, in vitro and in vivo blocking of tumor cells metastasis or stimulation of cell-adhesion, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155541). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

C57BL/6 splenocytes stained with M18/2 PE

Publications

Publications: Isobe, Yagita, Okumura et al.: "Specific acceptance of cardiac allograft after treatment with antibodies to ICAM-1 and LFA-1." in: Science (New York, N.Y.), Vol. 255, Issue 5048, pp. 1125-7, 1992 (PubMed).

Anderson, Springer: "Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins." in: Annual review of medicine, Vol. 38, pp. 175-94, 1987 (PubMed).

Sanchez-Madrid, Simon, Thompson et al.: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, 1983 (PubMed).

Zahalka, Naor: "Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness." in: International immunology, Vol. 6, Issue 6, pp. 917-24, 1994 (PubMed).

Ishida, Chused, Murakami et al.: "Antigen-specific cell conjugate formation and long-lasting calcium responses in recognition of Mls cellular superantigen by cloned murine T lymphocytes." in: Cellular immunology, Vol. 155, Issue 2, pp. 414-27, 1994 (PubMed).

Zahalka, Okon, Naor: "Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 10, pp. 4466-77, 1993 (PubMed).

Wilson, Ballantyne, Smith et al.: "Gene targeting yields a CD18-mutant mouse for study of inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1571-8, 1993 (PubMed).

Driessens, van Hulten, Zuurbier et al.: "Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct." in: Journal of leukocyte biology, Vol. 60, Issue 6, pp. 758-65, 1997 (PubMed).

Barlow, Langston, Matthews et al.: "CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes." in: The American journal of pathology, Vol. 165, Issue 6, pp. 1849-52, 2004 (PubMed).