CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody

Antigen: CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40)

Synonyms: p50, Bp50, CDW40, MGC9013, TNFRSF5, IGM, GP39, IMD3, TRAP, HIGM1, T-BAM, Tnfrsf5, AI326936, CD40

Clonality: Monoclonal (HM40-3)

Host: Hamster

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN155741

Additional Information

Alternative name: CD40

Immunogen: (BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: HM40-3

Description: CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions. Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34 hematopoietic progenitors Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production Synonyms: Bp50 Structure: TNFR superfamily, 48 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding, stimulation of mouse B cell and dendritic cell proliferation, and immunohistochemistry of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN155747). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

C57BL/6 splenocytes stained with HM40-3 biotin, then detected with Sav-PE

Publications

Publications: Clark, Ledbetter: "Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 12, pp. 4494-8, 1986 (PubMed).

Inaba, Inaba, Fukuba et al.: "Activation of thymic B cells by signals of CD40 molecules plus interleukin-10." in: European journal of immunology, Vol. 25, Issue 5, pp. 1244-8, 1995 (PubMed).

Kashiwada, Kaneko, Yagita et al.: "Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells." in: European journal of immunology, Vol. 26, Issue 7, pp. 1451-8, 1996 (PubMed).

Kaneko, Hirose, Abe et al.: "CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus." in: European journal of immunology, Vol. 26, Issue 12, pp. 3061-5, 1997 (PubMed).

Kawano, Cui, Koezuka et al.: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, 1997 (PubMed).

Ridge, Di Rosa, Matzinger: "A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell." in: Nature, Vol. 393, Issue 6684, pp. 474-8, 1998 (PubMed).

Akiba, Oshima, Takeda et al.: "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7058-66, 1999 (PubMed).

Morelli, OConnell, Khanna et al.: "Preferential induction of Th1 responses by functionally mature hepatic (CD8alpha- and CD8alpha+) dendritic cells: association with conversion from liver transplant tolerance to acute rejection." in: Transplantation, Vol. 69, Issue 12, pp. 2647-57, 2000 (PubMed).

Ma, Xu, An et al.: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006 (PubMed).

Yu, Siegel, Roeder: "Interaction of the B cell-specific transcriptional coactivator OCA-B and galectin-1 and a possible role in regulating BCR-mediated B cell proliferation." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15505-16, 2006 (PubMed).