HA-Tag antibody

Details for Product No. ABIN1558265, Supplier: Log in to see
Antigen
Reactivity
Tag
450
2
1
1
1
1
1
Host
Rat
180
178
40
34
19
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
360
201
115
109
81
70
53
40
15
13
11
7
7
5
4
4
4
4
3
2
2
1
1
1
1
1
1
Options
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Sequence YPYDVPDYA
Clone 7C9
Isotype IgG2a
Specificity The antibody recognizes an epitope located within the aminoterminal sequence YPYDVPDYA (residues 98-106) derived from the influenza hemagglutinin (HA) protein. The antibody recognizesthe HA tag sequence at either the N- or the C-terminus of the fusion protein.
Sensitivity Sensitivity of rat HA antibody 7C9 goes down to 10 ng of purified Sumo-HA and HA-GFP protein.
Purification Purifed antibody
Alternative Name HA Tag
Target Type Tag
Background Epitope tags are short peptide sequences of known epitopes, fused to recombinant proteins. Anti-tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. They are useful for specific visualization, immunoprecipitation or purification of recombinant proteins and protein complexes.
Research Area Tags/Labels
Application Notes Western blot: 1 µg/mL
Immunoprecipitation: recommended starting concentration 4 µg/IP
ELISA: not tested
IF: not tested
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0,09% Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN1558265) HEK293T cell extract expressing mCherry HA-Myc-His. Primary antibody: 1 µg/ml. Second...
 image for anti-HA-Tag antibody (ABIN1558265) Sensitivity of rat HA antibody goes down to 10 ng of purified Sumo-HA and HA-GFP prot...
Product cited in: Liu, Pedersen, Schultz-Larsen, Aguilar, Madriz-Ordeñana, Hovmøller, Thordal-Christensen: "The stripe rust fungal effector PEC6 suppresses pattern-triggered immunity in a host species-independent manner and interacts with adenosine kinases." in: The New phytologist, 2016 (PubMed).