I-A/I-E antibody

Antigen: I-A/I-E

Clonality: Monoclonal (M5-114-15-2)

Host: Rat

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN156271

Additional Information

Immunogen: Activated C57BL/6 mouse spleen cells

Format: Purified

Isotype: IgG2b, kappa chain  (Matching secondary antibodies)

Clone: M5-114-15-2

Description: The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A, I-A, I-A, I-E, and I-E MHC class II alloantigens. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The M5/114.15.2 antibody cross-reacts with the H-2 haplotypes. The M5/114.15.2 antibody has been reported to block antigen presentation. Distribution: B and activated T cells, APCs of the H-2 bearing mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Specificity: Reacts with a shared determinant on i-a

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular immunofluorescent staining, immunohistochemistry of frozen sections, and in vitro and in vivo blocking of antigen presentation or ligand binding. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156279). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4 °C.

Restrictions: For Research Use only

Images

C57BL/6 mouse splenocytes stained with M5/114 PE

Publications

Publications: Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, 1982 (PubMed).

Viville, Neefjes, Lotteau et al.: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, 1993 (PubMed).

Yamashita, Nagata, Tada et al.: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." in: International immunology, Vol. 5, Issue 9, pp. 1139-50, 1994 (PubMed).

Nelson, Hosier, Brady et al.: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2453-61, 1993 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Shi, Kaliyaperumal, Lu et al.: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." in: The Journal of experimental medicine, Vol. 187, Issue 3, pp. 367-78, 1998 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Kim, Noh, Kim et al.: "Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination." in: Experimental & molecular medicine, Vol. 36, Issue 5, pp. 428-43, 2004 (PubMed).

Luckashenak, Ryszkiewicz, Ramsey et al.: "The Src homology 2 domain-containing leukocyte protein of 76-kDa adaptor links integrin ligation with p44/42 MAPK phosphorylation and podosome distribution in murine dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5177-85, 2006 (PubMed).