I-Ek antibody (FITC)

Antigen: I-Ek

Clonality: Monoclonal (14-4-4S)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156508

Additional Information

Immunogen: C3H mouse skin graft and splenocytes

Isotype: IgG2a, IgG2a kappa, kappa chain

Clone: 14-4-4S

Description: The 14-4-4S antibody reacts with the I-E MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2, H-2, and H-2 haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2, H-2, H-2, and H-2 haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-E. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: I-E, MHC class II Structure: MHC class II

Specificity: Cross-reacts with h-2 haplotypes. mice of the h-2 haplotypes do not express i-e antigens. cross-reacts with rat mhc class ii antigen rt1d

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, in vitro induction of B-cell differentiation, in vitro blocking of antigen presentation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

C3H/He mouse splenocytes stained with 14-4-4S biotin and detected with Sav-PE

Publications

Publications: Harton, Litaker, Frelinger et al.: "Structure function analysis of the H-2 Abp gene." in: Immunogenetics, Vol. 34, Issue 6, pp. 358-65, 1992 (PubMed).

Ozato, Mayer, Sachs: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, 1980 (PubMed).

Roy, Aruffo, Ledbetter et al.: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, 1995 (PubMed).

Farr, DeRoos, Eastman et al.: "Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells." in: European journal of immunology, Vol. 26, Issue 12, pp. 3185-93, 1997 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Desmet, Gosset, Pajak et al.: "Selective blockade of NF-kappa B activity in airway immune cells inhibits the effector phase of experimental asthma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 9, pp. 5766-75, 2004 (PubMed).

Shih, Racz, Allen: "Differential MHC class II presentation of a pathogenic autoantigen during health and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3438-48, 2006 (PubMed).