I-Ek antibody (Alexa Fluor 488)

Antigen: I-Ek

Clonality: Monoclonal (14-4-4S)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Catalog no.: ABIN156513

Additional Information

Immunogen: C3H mouse skin graft and splenocytes

Isotype: IgG2a, IgG2a kappa, kappa chain

Clone: 14-4-4S

Description: The 14-4-4S antibody reacts with the I-E MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 14-4-4S antibody cross-reacts with cells from mice of the H-2, H-2, and H-2 haplotypes and the rat MHC class II antigen RT1D. Cells from mice of the H-2, H-2, H-2, and H-2 haplotypes do not express I-E antigen. The 14-4-4S antibody has been reported to block antigen presentation and induce differentiation of mouse cells bearing I-E. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: I-E, MHC class II Structure: MHC class II

Specificity: Cross-reacts with h-2 haplotypes. mice of the h-2 haplotypes do not express i-e antigens. cross-reacts with rat mhc class ii antigen rt1d

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, in vitro induction of B-cell differentiation, in vitro blocking of antigen presentation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

anti-I-Ek antibody (Alexa Fluor 488)

Publications

Publications: Harton, Litaker, Frelinger et al.: "Structure function analysis of the H-2 Abp gene." in: Immunogenetics, Vol. 34, Issue 6, pp. 358-65, 1992 (PubMed).

Ozato, Mayer, Sachs: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, 1980 (PubMed).

Roy, Aruffo, Ledbetter et al.: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, 1995 (PubMed).

Farr, DeRoos, Eastman et al.: "Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells." in: European journal of immunology, Vol. 26, Issue 12, pp. 3185-93, 1997 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Desmet, Gosset, Pajak et al.: "Selective blockade of NF-kappa B activity in airway immune cells inhibits the effector phase of experimental asthma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 9, pp. 5766-75, 2004 (PubMed).

Shih, Racz, Allen: "Differential MHC class II presentation of a pathogenic autoantigen during health and disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3438-48, 2006 (PubMed).