H-2Dk antibody (PE)

Antigen: H-2Dk

Clonality: Monoclonal (15-05-2005)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156522

Additional Information

Immunogen: C3H mouse splenocytes

Isotype: IgG2a, IgG2a kappa, kappa chain

Clone: 15-05-2005

Description: The 15-5-5 antibody reacts with the H-2D MHC class I alloantigen expressed on nucleated cells from mice of the H-2D haplotype. H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2K and with cells from mice of the H-2 haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported. Distribution: Nucleated cells of the H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

C3H/He mouse splenocytes stained with 15-5-5 FITC

Publications

Publications: Ozato, Mayer, Sachs: "Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 2, pp. 533-40, 1980 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Yamamoto, Fujihashi, Kawabata et al.: "A mucosal intranet: intestinal epithelial cells down-regulate intraepithelial, but not peripheral, T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2188-96, 1998 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).