H-2Dd antibody

Antigen: H-2Dd

Clonality: Monoclonal (34-2-12)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN156525

Additional Information

Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes

Format: Purified

Isotype: IgG2a, IgG2a kappa, kappa chain  (Matching secondary antibodies)

Clone: 34-2-12

Description: The 34-2-12 antibody recognizes alpha3 domain of H-2D MHC class I alloantigen expressed on nucleated cells from mice of the H-2D haplotype. H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Distribution: Nucleated cells of the H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, blocking of cell adhesion, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C.

Restrictions: For Research Use only

Images

BALB/c mouse splenocytes stained with 34-2-12 PE C3H/He mouse splenocytes stained with 34-2-12 PE

C3H/He mouse splenocytes stained with 34-2-12 PE

Publications

Publications: McCluskey, Bluestone, Coligan et al.: "Serologic and T cell recognition of truncated transplantation antigens encoded by in vitro deleted class I major histocompatibility genes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 4, pp. 1472-81, 1986 (PubMed).

McCluskey, Germain, Margulies: "Cell surface expression of an in vitro recombinant class II/class I major histocompatibility complex gene product." in: Cell, Vol. 40, Issue 2, pp. 247-57, 1985 (PubMed).

Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).

Daniels, Karlhofer, Seaman et al.: "A natural killer cell receptor specific for a major histocompatibility complex class I molecule." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 687-92, 1994 (PubMed).

Kane: "Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 1011-5, 1994 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Nikolic, Gardner, Scadden et al.: "Normal development in porcine thymus grafts and specific tolerance of human T cells to porcine donor MHC." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3402-7, 1999 (PubMed).

Ma, Xu, An et al.: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006 (PubMed).