H-2Db antibody (FITC)

Antigen: H-2Db

Clonality: Monoclonal (KH95)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156564

Additional Information

Immunogen: C57BL/10 mouse skin graft and splenocytes

Isotype: IgG2b, IgG2b kappa, kappa chain

Clone: KH95

Description: The KH95 antibody reacts with the H-2D MHC class I alloantigen expressed on nucleated cells from mice of the H-2D haplotype. H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported. Distribution: Nucleated cells of the H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, and Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

C57BL/6 splenocytes stained with KH95 PE BALB/c splenocytes stained with KH95 PE

BALB/c splenocytes stained with KH95 PE

Publications

Publications: Hasenkrug, Cory, Stimpfling: "Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region." in: Immunogenetics, Vol. 25, Issue 2, pp. 136-9, 1987 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Shao, Peng, Liao et al.: "A shared epitope of the interphotoreceptor retinoid-binding protein recognized by the CD4+ and CD8+ autoreactive T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1851-7, 2005 (PubMed).

Ponomarev, Shriver, Dittel: "CD40 expression by microglial cells is required for their completion of a two-step activation process during central nervous system autoimmune inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1402-10, 2006 (PubMed).