H-2Ld/H-2Db antibody (PE)

Antigen: H-2Ld/H-2Db

Clonality: Monoclonal (28-14-8)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156652

Additional Information

Immunogen: C3H.SW mouse splenocytes

Isotype: IgG2a, IgG2a kappa, kappa chain

Clone: 28-14-8

Description: The 28-14-8 antibody reacts with the H-2D MHC class I alloantigen expressed on nucleated cells from mice of the H-2D haplotype. H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-14-8 antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. The 28-14-8 antibody cross-reacts with the H-2L haplotype. Distribution: Nucleated cells of the H-2L/H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity, immunoprecipitation, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156654). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

C57BL/6 mouse splenocytes stained with 28-14-8 PE

Publications

Publications: Ozato, Hansen, Sachs: "Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 125, Issue 6, pp. 2473-7, 1981 (PubMed).

York, Rock: "Antigen processing and presentation by the class I major histocompatibility complex." in: Annual review of immunology, Vol. 14, pp. 369-96, 1996 (PubMed).

Bashuda, Seino, Ra et al.: "Lack of cognate help by CD4+ T cells and anergy of CD8+ T cells are the principal mechanisms for anti-leukocyte function-associated antigen-1/intercellular adhesion molecule-1-induced cardiac allograft tolerance." in: Transplantation, Vol. 63, Issue 1, pp. 113-8, 1997 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Marguet, Spiliotis, Pentcheva et al.: "Lateral diffusion of GFP-tagged H2Ld molecules and of GFP-TAP1 reports on the assembly and retention of these molecules in the endoplasmic reticulum." in: Immunity, Vol. 11, Issue 2, pp. 231-40, 1999 (PubMed).

Kafrouni, Brown, Thiele: "Virally infected hepatocytes are resistant to perforin-dependent CTL effector mechanisms." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1566-74, 2001 (PubMed).