H-2Kb/H-2Db antibody (Biotin)

Antigen: H-2Kb/H-2Db

Clonality: Monoclonal (28-08-2006)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156661

Additional Information

Immunogen: C3H.SW mouse splenocytes

Isotype: IgG2a, IgG2a kappa, kappa chain

Clone: 28-08-2006

Description: The 28-8-6 antibody reacts with the H-2K and H-2D MHC class I alloantigens expressed on nucleated cells from mice of the H-2K/H-2D haplotype. H-2K/H-2D is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2D MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes. Distribution: Nucleated cells of the H-2K/H-2D haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Restrictions: For Research Use only

Images

C57BL/6 mouse splenocytes stained with 28-8-6 PE

Publications

Publications: Allen, Fraser, Flyer et al.: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, 1986 (PubMed).

Evans, Margulies, Shykind et al.: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, 1983 (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, 1981 (PubMed).

Pappo, Torrey, Castriotta et al.: "Helicobacter pylori infection in immunized mice lacking major histocompatibility complex class I and class II functions." in: Infection and immunity, Vol. 67, Issue 1, pp. 337-41, 1999 (PubMed).

Bui, Carayannopoulos, Lanier et al.: "IFN-dependent down-regulation of the NKG2D ligand H60 on tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 2, pp. 905-13, 2006 (PubMed).