H-2Kk antibody (Biotin)

Antigen: H-2Kk

Clonality: Monoclonal (36-7-5)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156685

Additional Information

Immunogen: A.AL mouse splenocytes

Isotype: IgG2a, kappa chain

Clone: 36-7-5

Description: The 36-7-5 antibody reacts with the H-2K MHC class I alloantigen expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice. Distribution: Nucleated cells of the H-2k haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Specificity: Weakly cross-reacts with sjl/hsd mice splenocytes

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN156695). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Restrictions: For Research Use only

Images

C3H/He mouse splenocytes stained with 36-7-5 PE BALB/c mouse splenocytes stained with 36-7-5 PE

BALB/c mouse splenocytes stained with 36-7-5 PE

Publications

Publications: Ardehali, Laks, Drinkwater et al.: "Vascular cell adhesion molecule-1 is induced on vascular endothelia and medial smooth muscle cells in experimental cardiac allograft vasculopathy." in: Circulation, Vol. 92, Issue 3, pp. 450-6, 1995 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Goudeau, Huetz, Samson et al.: "IkappaBalpha/IkappaBepsilon deficiency reveals that a critical NF-kappaB dosage is required for lymphocyte survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 15800-5, 2003 (PubMed).

Johnson: "Murine oviduct epithelial cell cytokine responses to Chlamydia muridarum infection include interleukin-12-p70 secretion." in: Infection and immunity, Vol. 72, Issue 7, pp. 3951-60, 2004 (PubMed).