I-Ad antibody

Antigen: I-Ad

Clonality: Monoclonal (39-10-8)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Catalog no.: ABIN156697

Additional Information

Immunogen: (C3H x BALB/c)F1 mouse cells

Format: Purified

Isotype: IgG3, IgG3 kappa, kappa chain  (Matching secondary antibodies)

Clone: 39-10-8

Description: The 39-10-8 antibody with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s). Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.The antibody solution should be stored undiluted at 4°C.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Applications

Restrictions: For Research Use only

Images

BALB/c mouse splenocytes stained with 39-10-8 FITC C57BL/6 mouse splenocytes stained with 39-10-8 FITC

C57BL/6 mouse splenocytes stained with 39-10-8 FITC

Publications

Publications: Wall, Lorber, Loken et al.: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, 1983 (PubMed).

Hiramine, Nakagawa, Hojo: "Murine nursing thymic epithelial cell lines capable of inducing thymocyte apoptosis express the self-superantigen Mls-1a." in: Cellular immunology, Vol. 160, Issue 1, pp. 157-62, 1995 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Ridgway, Ito, Fasso et al.: "Analysis of the role of variation of major histocompatibility complex class II expression on nonobese diabetic (NOD) peripheral T cell response." in: The Journal of experimental medicine, Vol. 188, Issue 12, pp. 2267-75, 1999 (PubMed).

Wang, Castellaneta, De Creus et al.: "Heart, but not skin, allografts from donors lacking Flt3 ligand exhibit markedly prolonged survival time." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 5924-30, 2004 (PubMed).

Norian, Allen: "No intrinsic deficiencies in CD8+ T cell-mediated antitumor immunity with aging." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 2, pp. 835-44, 2004 (PubMed).

Ma, Xu, An et al.: "Vaccine with beta-defensin 2-transduced leukemic cells activates innate and adaptive immunity to elicit potent antileukemia responses." in: Cancer research, Vol. 66, Issue 2, pp. 1169-76, 2006 (PubMed).