I-Aq antibody (Biotin)

Antigen: I-Aq

Clonality: Monoclonal (KH116)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156713

Additional Information

Immunogen: B10.Q mouse splenocytes

Isotype: IgG2b, IgG2b kappa, kappa chain

Clone: KH116

Description: The KH116 antibody reacts with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH116 antibody cross-reacts with I-A and I-A, reactivity with other haplotypes has not been reported. Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Restrictions: For Research Use only

Images

anti-I-Aq antibody (Biotin)

Publications

Publications: Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Nakagawa, Brissette, Lira et al.: "Impaired invariant chain degradation and antigen presentation and diminished collagen-induced arthritis in cathepsin S null mice." in: Immunity, Vol. 10, Issue 2, pp. 207-17, 1999 (PubMed).

Zuo, Cullen, DeLay et al.: "A single-chain class II MHC-IgG3 fusion protein inhibits autoimmune arthritis by induction of antigen-specific hyporesponsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 5, pp. 2554-9, 2002 (PubMed).