H-2Kk antibody

Antigen: H-2Kk

Clonality: Monoclonal (AF3-12-1)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156850

Additional Information

Immunogen: A/J mouse splenocytes

Format: Purified

Isotype: IgG1, IgG1 kappa, kappa chain  (Matching secondary antibodies)

Clone: AF3-12-1

Description: The AF3-12.1 antibody reacts with the H-2K MHC class I alloantigen expressed on nucleated cells from mice of the H-2K haplotype. H-2K is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF3-12.1 antibody does not cross-react with other haplotypes (b, d, f, j, p, q, r, s, u, v). Distribution: Nucleated cells of the H-2k haplotype Function: Antigen presentation Synonyms: MHC class I Structure: MHC class I

Application Details

Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Product Information

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C.

Restrictions: For Research Use only

Images

anti-H-2Kk antibody

Publications

Publications: Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Yamamoto, Fujihashi, Kawabata et al.: "A mucosal intranet: intestinal epithelial cells down-regulate intraepithelial, but not peripheral, T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2188-96, 1998 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Wasowska, Qian, Cangello et al.: "Passive transfer of alloantibodies restores acute cardiac rejection in IgKO mice." in: Transplantation, Vol. 71, Issue 6, pp. 727-36, 2001 (PubMed).