I-Ab antibody (FITC)

Antigen: I-Ab

Clonality: Monoclonal (AF6-120-1)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN156858

Additional Information

Immunogen: C57BL/10J splenocytes

Isotype: IgG2a, IgG2a kappa, kappa chain

Clone: AF6-120-1

Description: The AF6-120.1 antibody reacts with the I-A MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2 and H-2 haplotypes, this antibody does not cross-react with other haplotypes (d, f, q, r, s). Distribution: B and activated T cells, APCs of H-2 mice Function: Antigen presentation Synonyms: MHC class II Structure: MHC class II

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunoprecipitation, and immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Product Information

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Images

anti-I-Ab antibody (FITC)

Publications

Publications: Cohn, Glimcher, Waldmann et al.: "Identification of functional regions on the I-Ab molecule by site-directed mutagenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 747-51, 1986 (PubMed).

Wall, Lorber, Loken et al.: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, 1983 (PubMed).

Watts: "Capture and processing of exogenous antigens for presentation on MHC molecules." in: Annual review of immunology, Vol. 15, pp. 821-50, 1997 (PubMed).

Pamer, Cresswell: "Mechanisms of MHC class I--restricted antigen processing." in: Annual review of immunology, Vol. 16, pp. 323-58, 1998 (PubMed).

Nakagawa, Brissette, Lira et al.: "Impaired invariant chain degradation and antigen presentation and diminished collagen-induced arthritis in cathepsin S null mice." in: Immunity, Vol. 10, Issue 2, pp. 207-17, 1999 (PubMed).

Buono, Come, Stavrakis et al.: "Influence of interferon-gamma on the extent and phenotype of diet-induced atherosclerosis in the LDLR-deficient mouse." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 23, Issue 3, pp. 454-60, 2003 (PubMed).

Wang, Castellaneta, De Creus et al.: "Heart, but not skin, allografts from donors lacking Flt3 ligand exhibit markedly prolonged survival time." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 5924-30, 2004 (PubMed).