His Tag antibody

Details for Product No. ABIN1573881, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
24
22
14
3
2
Host
Rabbit
435
183
27
23
4
4
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
554
385
167
107
99
77
69
26
13
11
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen His epitope tag peptide HHHHHH conjugated - KLH
Sequence HHHHHH
Isotype IgG
Specificity Rabbit Anti-His-tag Polyclonal Antibody specifically reacts with fusion proteins containing either amino- or carboxy-terminal His epitope tags.
Purification Immunoaffinity chromatography
Alternative Name His-tag
Target Type Tag
Background Rabbit Anti-His-tag Polyclonal Antibody recognizes the His tag placed at N-terminal, C-terminal, and internal regions of targeted proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN1573881) Western blot analysis of His-fusion protein (MW~33 kD) using 1 µg/mL Rabbit Anti-His-...
Western Blotting (WB) image for anti-His Tag antibody (ABIN1573881) Western blot analysis of His-fusion protein (MW~91 kD) using 1 µg/mL Rabbit Anti-His-...
Western Blotting (WB) image for anti-His Tag antibody (ABIN1573881) Western blot analysis of His-fusion protein (MW~91 kD) using 1 µg/mL Rabbit Anti-His-...
Product cited in: Dan, Kang, Duan, Wang: "A cell-free system toward deciphering the post-translational modification barcodes of Oct4 in different cellular contexts." in: Biochemical and biophysical research communications, Vol. 456, Issue 3, pp. 714-20, 2015 (PubMed).

Zhao, Zhou, Yao, Wan, Cao, Zhang, Zhao, Li, Zhou, Li, Wei, Zhang, French, Dekker, Yang, Fisher, Tucker, Guo: "Foxp1/2/4 regulate endochondral ossification as a suppresser complex." in: Developmental biology, Vol. 398, Issue 2, pp. 242-54, 2015 (PubMed).

Chen, Yang, Pang, Peng, Dong, Liu, Ouyang, Ren: "Expression, purification and antibody preparation using different constructs of PCV2 capsid protein." in: International journal of biological macromolecules, Vol. 67, pp. 289-94, 2014 (PubMed).

Baldwin, Yamodo, Ranjan, Li, Mines, Marinkovic, Hanada, Oh, Chishti: "Human erythrocyte band 3 functions as a receptor for the sialic acid-independent invasion of Plasmodium falciparum. Role of the RhopH3-MSP1 complex." in: Biochimica et biophysica acta, Vol. 1843, Issue 12, pp. 2855-70, 2014 (PubMed).

Steiner, Lori, Boehm, Jenal: "Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction." in: The EMBO journal, Vol. 32, Issue 3, pp. 354-68, 2013 (PubMed).

Wood, Iverson-Cabral, Patton, Cummings, Cosgrove Sweeney, Totten: "Persistence, immune response, and antigenic variation of Mycoplasma genitalium in an experimentally infected pig-tailed macaque (Macaca nemestrina)." in: Infection and immunity, Vol. 81, Issue 8, pp. 2938-51, 2013 (PubMed).

Raimunda, Subramanian, Stemmler, Argüello: "A tetrahedral coordination of Zinc during transmembrane transport by P-type Zn(2+)-ATPases." in: Biochimica et biophysica acta, Vol. 1818, Issue 5, pp. 1374-7, 2012 (PubMed).

Tosca, Glass, Bronchain, Philippe, Ciapa: "PLC?, G-protein of the G?q type and cADPr pathway are associated to trigger the fertilization Ca2+ signal in the sea urchin egg." in: Cell calcium, Vol. 52, Issue 5, pp. 388-96, 2012 (PubMed).

Sundlisaeter, Edelmann, Hol, Sponheim, Küchler, Weiss, Udalova, Midwood, Kasprzycka, Haraldsen: "The alarmin IL-33 is a notch target in quiescent endothelial cells." in: The American journal of pathology, Vol. 181, Issue 3, pp. 1099-111, 2012 (PubMed).

Rosadini, Gawronski, Raimunda, Argüello, Akerley: "A novel zinc binding system, ZevAB, is critical for survival of nontypeable Haemophilus influenzae in a murine lung infection model." in: Infection and immunity, Vol. 79, Issue 8, pp. 3366-76, 2011 (PubMed).

Sandee, Miller: "High-yield expression of a catalytically active membrane-bound protein: human P450 oxidoreductase." in: Endocrinology, Vol. 152, Issue 7, pp. 2904-8, 2011 (PubMed).

Yang, Hatton-Ellis, Simpson: "The kinase Sgg modulates temporal development of macrochaetes in Drosophila by phosphorylation of Scute and Pannier." in: Development (Cambridge, England), Vol. 139, Issue 2, pp. 325-34, 2011 (PubMed).

Kaufer, Smejkal, Osterrieder: "The varicella-zoster virus ORFS/L (ORF0) gene is required for efficient viral replication and contains an element involved in DNA cleavage." in: Journal of virology, Vol. 84, Issue 22, pp. 11661-9, 2010 (PubMed).

Lee, Park, Takahashi, Wang: "The association of AMPK with ULK1 regulates autophagy." in: PLoS ONE, Vol. 5, Issue 11, pp. e15394, 2010 (PubMed).

Medici, Shore, Lounev, Kaplan, Kalluri, Olsen: "Conversion of vascular endothelial cells into multipotent stem-like cells." in: Nature medicine, Vol. 16, Issue 12, pp. 1400-6, 2010 (PubMed).

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