Fibrillarin antibody (AA 50-100)

Catalog No. ABIN1574130

Product Details anti-Fibrillarin Antibody

Target: Fibrillarin (FBL)

Binding Specificity: AA 50-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rabbit Anti-Fibrillarin Polyclonal Antibody detects endogenous levels of human Fibrillarin.Positive Control: Hela and HEK293.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 50-100 of human Fibrillarin protein .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Fibrillarin antibody (ABIN1574130)

Berkovits, Wolgemuth: "The first bromodomain of the testis-specific double bromodomain protein Brdt is required for chromocenter organization that is modulated by genetic background." in: Developmental biology, Vol. 360, Issue 2, pp. 358-68, (2011) (PubMed).

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: Fibrillarin (FBL Products)

Background: Fibrillarin is an excellent marker for the nucleolus. As a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle, it is thought to participate in the first step in processing preribosomal RNA. Fibrillarin protein contains an amino-terminal domain that is rich in glycine and arginine residues (termed the GAR domain), a central RNA-binding domain comprising an RNP-2 like consensus sequence, and a C-terminal alpha-helical domain. Fibrillarin binds to U3, U8, and U13 small nuclear RNAs. It associates with several other structural proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2-O-ribose methylation of targeted nucleotides within the rRNA sequence. Rabbit Anti-Fibrillarin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 50-100 of human Fibrillarin protein (Swiss Prot: P22087).

Pathways: Ribonucleoside Biosynthetic Process