TUBB antibody

Catalog No. ABIN1574254

Product Details anti-TUBB Antibody

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Fish, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.

Cross-Reactivity (Details): beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.

Purification: Protein A affinity column

Immunogen: purified human recombinant full-length β-tubulin protein

Clone: 2G7D4

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Flow cytometry:1 µg for 1 x 106 cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-TUBB antibody (ABIN1574254)

Fisher, Resnick, De, Acevedo, Lu, Schroeder, Nicholson: "Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway." in: Journal of Alzheimer's disease : JAD, Vol. 55, Issue 1, pp. 391-410, (2016) (PubMed).

Huang, Nishi, Li, Ho, Dong, Chang, Chang: "Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain." in: The FEBS journal, Vol. 281, Issue 7, pp. 1773-83, (2014) (PubMed).

Sukthankar, Avila, Whitaker, Iwamoto, Morgenstern, Apostolidis, Liu, Hanzlik, Dadachova, Tomich: "Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes." in: Biochimica et biophysica acta, Vol. 1838, Issue 9, pp. 2296-305, (2014) (PubMed).

Infanger, Cho, Lopez, Mohanan, Liu, Gursel, Boockvar, Fischbach: "Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche." in: Cancer research, Vol. 73, Issue 23, pp. 7079-89, (2013) (PubMed).

Wang, Chatterjee, Jeon, Akerman, Vander Heiden, Cantley, Krainer: "Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons." in: Journal of molecular cell biology, Vol. 4, Issue 2, pp. 79-87, (2012) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin (TUBB Products)

Synonyms: M40 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, XLOT antibody, m40 antibody, ok/sw-cl.56 antibody, tubb1 antibody, tubb5 antibody, TUBB2A antibody, TUBB2B antibody, TUBB antibody, 1t antibody, B1t antibody, BETA 56D antibody, CG9277 antibody, DTB2 antibody, Dmbeta1 antibody, Dmel\\CG9277 antibody, T antibody, Tub antibody, Tubulin antibody, beta antibody, beta-Tub antibody, beta-Tub56D antibody, beta-tub antibody, beta-tubulin56D antibody, beta1 antibody, beta1-Tubulin antibody, beta1-tub antibody, beta1Tub antibody, beta1t antibody, beta1tub antibody, beta56D antibody, betaTub antibody, betaTub1 antibody, beta[[1]] tubulin antibody, beta[[1]]-tubulin antibody, betatub(56D) antibody, 143391_i_at antibody, 3t antibody, B3t antibody, BETA 60D antibody, CG3401 antibody, D.m.BETA-60D antibody, DTB3 antibody, Dmbeta3 antibody, Dmel\\CG3401 antibody, Tub60D antibody, beta-Tub60D antibody, beta-Tub6D antibody, beta3 antibody, beta3 TU antibody, beta3-Tub antibody, beta3-tubulin antibody, beta3TUB antibody, beta3Tub antibody, beta3t antibody, beta60C antibody, betaTub3 antibody, betaTub60C antibody, beta[[3]] tubulin antibody, beta[[3]]-Tub antibody, beta[[3]]-tubulin antibody, betatub60D antibody, p50 antibody, p50/tubulin antibody, p53 antibody, p53/tubulin antibody, 2t antibody, B2t antibody, BETA 85D antibody, BETA2 antibody, CG9359 antibody, D.m.BETA-85D antibody, DTB4 antibody, Dmbeta2 antibody, Dmel\\CG9359 antibody, beta(2)Tu antibody, beta(2)Tub antibody, beta-Tub85D antibody, beta-tub85D antibody, beta2 antibody, beta2-tub antibody, beta2t antibody, beta2tub antibody, beta85D antibody, betaTub2 antibody, beta[[2]]-tubulin antibody, ms(3)KK[D] antibody, 4t antibody, B4t antibody, BETA 98B antibody, CG4869 antibody, DTB1 antibody, Dmbeta4 antibody, Dmel\\CG4869 antibody, beta-Tub97EF antibody, beta4 antibody, beta4t antibody, beta97F antibody, betaTub4 antibody, betaTub98BC antibody, betaTub98C antibody, Tub1 antibody, bmtub1 antibody, Tub3 antibody, bmtub3 antibody, Tub2 antibody, bmtub2 antibody, Tub4 antibody, bmtub4 antibody, LOC100101153 antibody, tubulin beta class I antibody, putative cobalt-zinc-cadmium outer membrane resistance protein antibody, Tubulin beta chain antibody, tubulin beta-2A chain antibody, beta-Tubulin at 56D antibody, beta-Tubulin at 60D antibody, beta-Tubulin at 85D antibody, beta-Tubulin at 97EF antibody, beta-tubulin antibody, tubulin beta 6 class V antibody, beta tubulin antibody, tubulin beta class I L homeolog antibody, tubulin, beta 5 class I antibody, TUBB antibody, czcC antibody, tbb-6 antibody, tubb antibody, LOC462399 antibody, betaTub56D antibody, betaTub60D antibody, betaTub85D antibody, betaTub97EF antibody, Tub1 antibody, Tub3 antibody, Tub2 antibody, Tub4 antibody, TVAG_525430 antibody, TVAG_062880 antibody, TVAG_456920 antibody, LOC100101153 antibody, TUBB6 antibody, Tb927.1.2330 antibody, Tb927.1.2350 antibody, Tb927.1.2370 antibody, Tb927.1.2390 antibody, tubb.L antibody, Tubb antibody, LOC693049 antibody, LOC443015 antibody, Tubb5 antibody

Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (alpha) and beta (beta) chains. beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots. beta-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length beta-tubulin protein.

Pathways: Microtubule Dynamics, M Phase