TUBB antibody (Tubulin, beta)

Details for Product anti-TUBB Antibody No. ABIN1574254, Supplier: Log in to see
Antigen
  • XLOT
  • m40
  • ok/sw-cl.56
  • tubb1
  • tubb5
  • 1t
  • B1t
  • BETA 56D
  • CG9277
  • DTB2
  • Dmbeta1
  • Dmel\\CG9277
  • T
  • Tub
  • Tubulin
  • beta
  • beta-Tub
  • beta-Tub56D
  • beta-tub
  • beta-tubulin56D
  • beta1
  • beta1-Tubulin
  • beta1-tub
  • beta1Tub
  • beta1t
  • beta1tub
  • beta56D
  • betaTub
  • betaTub1
  • beta[[1]] tubulin
  • beta[[1]]-tubulin
  • betatub(56D)
  • 143391_i_at
  • 3t
  • B3t
  • BETA 60D
  • CG3401
  • D.m.BETA-60D
  • DTB3
  • Dmbeta3
  • Dmel\\CG3401
  • Tub60D
  • beta-Tub60D
  • beta-Tub6D
  • beta3
  • beta3 TU
  • beta3-Tub
  • beta3-tubulin
  • beta3TUB
  • beta3Tub
  • beta3t
  • beta60C
  • betaTub3
  • betaTub60C
  • beta[[3]] tubulin
  • beta[[3]]-Tub
  • beta[[3]]-tubulin
  • betatub60D
  • p50
  • p50/tubulin
  • p53
  • p53/tubulin
  • 2t
  • B2t
  • BETA 85D
  • BETA2
  • CG9359
  • D.m.BETA-85D
  • DTB4
  • Dmbeta2
  • Dmel\\CG9359
  • beta(2)Tu
  • beta(2)Tub
  • beta-Tub85D
  • beta-tub85D
  • beta2
  • beta2-tub
  • beta2t
  • beta2tub
  • beta85D
  • betaTub2
  • beta[[2]]-tubulin
  • ms(3)KK[D]
  • tubB3
  • tubulin
  • Tub1
  • bmtub1
  • Tub3
  • bmtub3
  • Tub2
  • bmtub2
  • Tub4
  • bmtub4
  • LOC100101153
  • beta-5
  • tubb2a
  • tubb4
  • tub5
  • 4t
  • B4t
  • BETA 98B
  • CG4869
  • DTB1
  • Dmbeta4
  • Dmel\\CG4869
  • beta-Tub97EF
  • beta4
  • beta4t
  • beta97F
  • betaTub4
  • betaTub98BC
  • betaTub98C
  • TUBB2A
  • TUBB
  • TUBB5
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • AA408537
  • AI596182
  • B130022C14Rik
  • M(beta)5
  • TUBB2B
  • M40
  • OK/SW-cl.56
  • TUBB1
  • tubulin, beta-like
  • Tubulin, Beta
  • tubulin, beta class I
  • beta-Tubulin at 56D
  • beta-Tubulin at 60D
  • beta-Tubulin at 85D
  • beta-tubulin
  • tubulin beta
  • tubulin, beta 4A class IVa
  • tubulin, beta 2A class IIa
  • aarF domain containing kinase 5
  • putative cobalt-zinc-cadmium outer membrane resistance protein
  • beta tubulin
  • beta-5 tubulin
  • beta-Tubulin at 97EF
  • tubulin, beta
  • tubulin, beta 4A class IVA
  • tubulin, beta 5 class I
  • tubulin, beta 6 class V
  • LOC100350045
  • tbb-6
  • tubb
  • betaTub56D
  • betaTub60D
  • betaTub85D
  • tubB3
  • Tub1
  • Tub3
  • Tub2
  • Tub4
  • TVAG_525430
  • TVAG_062880
  • TVAG_456920
  • TUB3
  • TUB2
  • LOC100101153
  • tubb4a
  • TUBB2A
  • ADCK5
  • czcC
  • Tb927.1.2330
  • Tb927.1.2350
  • Tb927.1.2370
  • Tb927.1.2390
  • LOC100689088
  • TUBB5
  • LOC693049
  • LOC443015
  • betaTub97EF
  • TUBB
  • Tubb4a
  • Tubb5
  • TUBB6
Alternatives
anti-Pig (Porcine) TUBB antibody for Western Blotting
Reactivity
Fish, Mouse (Murine), Rat (Rattus), Rabbit, Pig (Porcine), Cow (Bovine), Goat, Chicken, Human
367
262
249
70
69
63
44
33
29
28
22
17
16
15
12
11
11
11
10
10
9
9
7
6
6
6
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
235
166
1
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB antibody is un-conjugated
14
9
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
350
145
121
113
110
83
27
21
19
17
8
7
3
3
2
2
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen purified human recombinant full-length β-tubulin protein
Clone 2G7D4
Isotype IgG1
Specificity Beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.
Cross-Reactivity (Details) beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.
Purification Protein A affinity column
Alternative Name beta-Tubulin (TUBB Antibody Abstract)
Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (alpha) and beta (beta) chains. beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots. beta-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length beta-tubulin protein.
Pathways Microtubule Dynamics, M Phase
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Flow cytometry:1 µg for 1 x 106 cells
Restrictions For Research Use only
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Product cited in: Huang, Nishi, Li, Ho, Dong, Chang, Chang: "Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain." in: The FEBS journal, Vol. 281, Issue 7, pp. 1773-83, 2014 (PubMed).

Sukthankar, Avila, Whitaker, Iwamoto, Morgenstern, Apostolidis, Liu, Hanzlik, Dadachova, Tomich: "Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes." in: Biochimica et biophysica acta, Vol. 1838, Issue 9, pp. 2296-305, 2014 (PubMed).

Infanger, Cho, Lopez, Mohanan, Liu, Gursel, Boockvar, Fischbach: "Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche." in: Cancer research, Vol. 73, Issue 23, pp. 7079-89, 2013 (PubMed).

Wang, Chatterjee, Jeon, Akerman, Vander Heiden, Cantley, Krainer: "Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons." in: Journal of molecular cell biology, Vol. 4, Issue 2, pp. 79-87, 2012 (PubMed).

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