CD8a antibody (Alexa Fluor 488)

Antigen: CD8a

Clonality: Monoclonal (HIT8a)

Host: Mouse

Reactivity: Human

Conjugate: Alexa Fluor 488

Application: Flow Cytometry (FACS)

Catalog no.: ABIN157586

Additional Information

Isotype: IgG1, kappa

Clone: HIT8a

Description: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the alpha3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck. Distribution: Majority of thymocytes, T cell subset, NK cells Function: MHC class I co-receptor, thymic differentiation, T cell activation Synonyms: T8, Leu2 Structure: Ig superfamily, homodimer or heterodimer with CD8ß, 32-34 kD

Application Details

Application Notes: Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections. The HIT8a antibody does not block the binding of RPA-T8 antibody to CD8a. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Purification: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Human peripheral blood lymphocytes stained with HIT8a Alexa Fluor(R) 488