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anti-CD8a antibody (Alexa Fluor 488)

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Image for anti-CD8a antibody (Alexa Fluor 488)
Antigen: CD8a
Clonality: Monoclonal (HIT8a)
Application: Flow Cytometry (FACS)
Reactivity: Human
Host: Mouse
Conjugate: Alexa Fluor 488
Quantity: 100 tests
Price: 332,51 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN157586
Availability: Ships within 5 to 7 Business days
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Additional Information  » anti-CD8a antibody (Alexa Fluor 488)

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Antigen CD8a
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1, kappa »Matching secondary antibodies
Description CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the alpha3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck. Distribution: Majority of thymocytes, T cell subset, NK cells Function: MHC class I co-receptor, thymic differentiation, T cell activation Synonyms: T8, Leu2 Structure: Ig superfamily, homodimer or heterodimer with CD8ß, 32-34 kD
Clone HIT8a

Application Details  » anti-CD8a antibody (Alexa Fluor 488)

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Application Flow Cytometry (FACS)
» Click here to view 5 available protocols!
Application Notes Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections. The HIT8a antibody does not block the binding of RPA-T8 antibody to CD8a. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Conjugate Alexa Fluor 488
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Research Area CD Antigens
Restrictions For Research Use only