CD8a antibody (Biotin)

Antigen: CD8a

Clonality: Monoclonal (RPA-T8)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Catalog no.: ABIN157594

Additional Information

Isotype: IgG1, kappa chain

Clone: RPA-T8

Description: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the alpha3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck. Distribution: Majority of thymocytes, T cell subset, NK cells Function: MHC class I co-receptor, thymic differentiation, T cell activation Synonyms: T8, Leu2 Structure: Ig superfamily, homodimer or heterodimer with CD8beta, 32-34 kD

Application Details

Application Notes: Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, and costimulation of T cell responses. The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157608). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume or per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Human peripheral blood lymphocytes stained with biotinylated RPA-T8, followed by Sav-PE

Publications

Publications: Mack, Tucker, Sokol et al.: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004 (PubMed).

Magidovich, Orr, Fass et al.: "Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 32, pp. 13022-7, 2007 (PubMed).