Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) antibody (Pacific Blue)

Antigen: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)

Synonyms: CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA

Clonality: Monoclonal (3G8)

Host: Mouse

Reactivity: Human

Conjugate: Pacific Blue

Application: Flow Cytometry (FACS)

Catalog no.: ABIN157750

Additional Information

Alternative name: CD16

Isotype: IgG1, kappa chain

Clone: 3G8

Description: CD16 is known as low affinity IgG receptor III (FcgammaRIII). It is expressed as two distinct (CD16a and CD16b) forms. CD16a (FcgammaRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcgammaRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. It is over 95% homologous to CD16a in extracellular domain and is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex that functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). The 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Distribution: NK cells, activated monocytes, macrophages, neutrophils Function: Low affinity IgG Fc receptor, phagocytosis, ADCC Synonyms: FcgammaRIII Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form, 48 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation, stimulation of NK cell proliferation, blocking of phagocytosis, and blocking of immunoglobulin binding to FcgammaRIII. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157740). This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Purification: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue(TM) under optimal conditions. The solution is free of unconjugated Pacific Blue(TM).

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Publications

Publications: Wirthmueller, Kurosaki, Murakami et al.: "Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain." in: The Journal of experimental medicine, Vol. 175, Issue 5, pp. 1381-90, 1992 (PubMed).

Stroncek, Skubitz, Plachta et al.: "Alloimmune neonatal neutropenia due to an antibody to the neutrophil Fc-gamma receptor III with maternal deficiency of CD16 antigen." in: Blood, Vol. 77, Issue 7, pp. 1572-80, 1991 (PubMed).

Hoshino, Oshimi, Teramura et al.: "Activation via the CD3 and CD16 pathway mediates interleukin-2-dependent autocrine proliferation of granular lymphocytes in patients with granular lymphocyte proliferative disorders." in: Blood, Vol. 78, Issue 12, pp. 3232-40, 1992 (PubMed).

Fleit, Wright, Unkeless: "Human neutrophil Fc gamma receptor distribution and structure." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 10, pp. 3275-9, 1982 (PubMed).

Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, 1996 (PubMed).

Edberg, Kimberly: "Cell type-specific glycoforms of Fc gamma RIIIa (CD16): differential ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 8, pp. 3849-57, 1997 (PubMed).

Hober, Chehadeh, Weill et al.: "Circulating and cell-bound antibodies increase coxsackievirus B4-induced production of IFN-alpha by peripheral blood mononuclear cells from patients with type 1 diabetes." in: The Journal of general virology, Vol. 83, Issue Pt 9, pp. 2169-76, 2002 (PubMed).

Holl, Hemmerter, Burrer et al.: "Involvement of Fc gamma RI (CD64) in the mechanism of HIV-1 inhibition by polyclonal IgG purified from infected patients in cultured monocyte-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 10, pp. 6274-83, 2004 (PubMed).