Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (Pacific Blue)

Antigen: Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1)

Synonyms: B1, S7, Bp35, CD20, MS4A2, LEU-16, MGC3969, Cd20, Ly-44, Ms4a2, AA960661, FcRB, Fce1b, Ms4a1, Fcer1b, Fcrbeta, FCIGA, MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like

Clonality: Monoclonal (2H7)

Host: Mouse

Reactivity: Human

Conjugate: Pacific Blue

Application: Flow Cytometry (FACS)

Catalog no.: ABIN157810

Additional Information

Alternative name: CD20

Isotype: IgG2b, kappa chain

Clone: 2H7

Description: CD20 is a 33-37 kD four transmembrane spanning protein also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated in activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2 conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complex with MHC class I and II, CD53, CD81, and CD82. Distribution: B cell, T cell subsets Function: B cell activation Synonyms: B1, Bp35 Structure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Purification: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue(TM) under optimal conditions. The solution is free of unconjugated Pacific Blue(TM).

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (Pacific Blue)

Publications

Publications: Tedder, Engel: "CD20: a regulator of cell-cycle progression of B lymphocytes." in: Immunology today, Vol. 15, Issue 9, pp. 450-4, 1994 (PubMed).

Hultin, Hausner, Hultin et al.: "CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes." in: Cytometry, Vol. 14, Issue 2, pp. 196-204, 1993 (PubMed).

Polyak, Deans: "Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure." in: Blood, Vol. 99, Issue 9, pp. 3256-62, 2002 (PubMed).

Mack, Tucker, Sokol et al.: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004 (PubMed).