CD41 (ITGA2B) antibody

Antigen: CD41 (ITGA2B)

Synonyms: GTA, CD41, GP2B, HPA3, CD41B, GPIIb

Clonality: Monoclonal (HIP8)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN157960

Additional Information

Alternative name: CD41

Format: Purified

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Clone: HIP8

Description: CD41 is a 125/25 kD alpha subunit of the gpIIb/IIIa (CD41/CD61) complex. CD41 is a heterodimer composed of a heavy chain (gpIIbalpha) and light chain (gpIIbbeta) linked by a single disulfide bond. It is a member of the integrin family primarily expressed on platelets and megakaryocytes. CD41 has been reported to be involved with platelet aggregation and platelet attachment to the ECM. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, and thrombin. The HIP8 antibody has been reported to block the activation of platelets by various stimuli, including collagen, and ADP. Distribution: Platelets, megakaryocytes Function: Platelet aggregation, platelet attachment to extracellular matrix Synonyms: gpIIb Structure: Integrin family, alpha subunit of CD41/CD61 (GPIIb-IIIa) complex, 125/22 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and blocking of platelet aggregation. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Human peripheral blood platelets stained with HIP8, followed by anti-mouse IgGs FITC

Publications

Publications: Denzin, Cresswell: "HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading." in: Cell, Vol. 82, Issue 1, pp. 155-65, 1995 (PubMed).

Riberdy, Avva, Geuze et al.: "Transport and intracellular distribution of MHC class II molecules and associated invariant chain in normal and antigen-processing mutant cell lines." in: The Journal of cell biology, Vol. 125, Issue 6, pp. 1225-37, 1994 (PubMed).

Denzin, Hammond, Cresswell: "HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2153-65, 1997 (PubMed).

McCarty, Mousa, Bray et al.: "Immobilized platelets support human colon carcinoma cell tethering, rolling, and firm adhesion under dynamic flow conditions." in: Blood, Vol. 96, Issue 5, pp. 1789-97, 2000 (PubMed).