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GAP43 antibody

GAP43 Reactivity: Rat, Mouse WB, IHC, IF, ICC Host: Mouse Monoclonal 1E3 unconjugated
Catalog No. ABIN1580421
  • Target See all GAP43 Antibodies
    GAP43 (Growth Associated Protein 43 (GAP43))
    Reactivity
    • 107
    • 99
    • 94
    • 19
    • 9
    • 8
    • 7
    • 6
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat, Mouse
    Host
    • 94
    • 19
    • 7
    • 2
    • 1
    Mouse
    Clonality
    • 95
    • 28
    Monoclonal
    Conjugate
    • 76
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GAP43 antibody is un-conjugated
    Application
    • 77
    • 38
    • 32
    • 27
    • 27
    • 26
    • 24
    • 24
    • 19
    • 17
    • 16
    • 4
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Purification
    affinity purified antibody
    Clone
    1E3
    Isotype
    IgG1
    Top Product
    Discover our top product GAP43 Primary Antibody
  • Application Notes
    The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    The antibody solution is affinity purified from tissue culture supernatant and is at concentration of 1mg/ml in phosphate buffered saline preparation containing 10 mM sodium azide preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4°C short term or -20°C long term.
  • Target
    GAP43 (Growth Associated Protein 43 (GAP43))
    Alternative Name
    GAP43 (GAP43 Products)
    Synonyms
    B-50 antibody, PP46 antibody, Basp2 antibody, GAP-43 antibody, b-50 antibody, gap-43 antibody, gap43b antibody, pp46 antibody, cb310 antibody, gap antibody, gap43 antibody, gap43a antibody, growth associated protein 43 antibody, growth associated protein 43 L homeolog antibody, growth associated protein 43 S homeolog antibody, GAP43 antibody, Gap43 antibody, gap43.L antibody, gap43 antibody, gap43.S antibody
    Background
    GAP43 is very abundant nervous system protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14KDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomolously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2 and tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications. The HGNC name for this protein is GAP43.
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