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Vimentin antibody

VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Mammalian WB, IHC, IF, ICC Host: Mouse Monoclonal 2D1 unconjugated
Catalog No. ABIN1580468
  • Target See all Vimentin (VIM) Antibodies
    Vimentin (VIM)
    Reactivity
    • 332
    • 125
    • 124
    • 60
    • 57
    • 43
    • 42
    • 36
    • 29
    • 21
    • 20
    • 13
    • 13
    • 8
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Pig, Cow, Mammalian
    Host
    • 190
    • 128
    • 9
    • 6
    • 4
    • 4
    • 2
    Mouse
    Clonality
    • 198
    • 144
    Monoclonal
    Conjugate
    • 199
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Vimentin antibody is un-conjugated
    Application
    • 281
    • 132
    • 98
    • 97
    • 96
    • 65
    • 48
    • 40
    • 39
    • 28
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Purification
    affinity purified antibody
    Clone
    2D1
    Isotype
    IgG2a
    Top Product
    Discover our top product VIM Primary Antibody
  • Application Notes
    The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4°C short term or -20°C long term.
  • Target
    Vimentin (VIM)
    Alternative Name
    Vimentin (VIM Products)
    Synonyms
    CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
    Background
    Vimentin is a protein subunit of 10 nm or intermediate filaments, which are major components of the cytoskeleton in most cell types. Vimentin is the major intemediate filament subunit found in mesenchymal cells, and was first named and characterized in a collaborative study from the labs of German scientists Werner Franke and Klaus Weber. The name derives from the Latin Vimentum, meaning arrays of flexible rods such as in lattices, filigrees and wicker-work, which describes the intermediate filament network quite well. Vimentin is also found in many cell types in tissue culture, most notably fibroblasts, and in developing neuronal and astrocytic precursor cells in the central nervous system. Many cell lines such as Hek293, HeLa, 3T3 and Cos cells contain prominent vimentin networks. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in astrocytes, ependymal cells and neural stem cells), with desmin (in muscle and endothelial cells) and neurofilament proteins (in developing neurons). A E151K point mutation in the vimentin gene was shown to be causative of an autosomal dominant pulverulent cataract disease, but so far only in a single patient. Vimentin is a major protein of eye lens and cornea, and this mutation renders the molecule unable assemble into normal 10 nm filaments. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli. The same immunogen was used to produce our other monoclonal antibody to vimentin MCA-2A52. We also market a very popular chicken polyclonal antibody to vimentin, CPCA-Vim. The HGNC name for this protein is VIM.
    Pathways
    Caspase Cascade in Apoptosis
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