CD56 (NCAM) antibody

Antigen: CD56 (NCAM)

Clonality: Monoclonal (MEM-188)

Host: Mouse

Reactivity: Human, Monkey, Mouse (Murine), Pig (Porcine)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN158062

Additional Information

Immunogen: KG-1 human acute myelogenous leukemia cell line

Format: Purified

Isotype: IgG2a, kappa chain  (Matching secondary antibodies)

Clone: MEM-188

Description: CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate. Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias Function: Adhesion Synonyms: NCAM, Leu-19, NKH1 Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C.

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Human peripheral blood lymphocytes stained with purified MEM-188, followed by anti-mouse IgGs FITC

Publications

Publications: Lanier, Chang, Azuma et al.: "Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 12, pp. 4421-6, 1991 (PubMed).

Hemperly, DeGuglielmo, Reid: "Characterization of cDNA clones defining variant forms of human neural cell adhesion molecule N-CAM." in: Journal of molecular neuroscience : MN, Vol. 2, Issue 2, pp. 71-8, 1991 (PubMed).

Cremer, Lange, Christoph et al.: "Inactivation of the N-CAM gene in mice results in size reduction of the olfactory bulb and deficits in spatial learning." in: Nature, Vol. 367, Issue 6462, pp. 455-9, 1994 (PubMed).