zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Clonality: Monoclonal (1E7-2)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Intracellular Flow Cytometry (ICFC), Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN158747

Additional Information

Alternative name: ZAP-70

Immunogen: GST-fusion protein corresponding to residues 282-307 of human ZAP-70

Format: Purified

Isotype: IgG1, IgG1 kappa  (Matching secondary antibodies)

Clone: 1E7-2

Description: ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation. Distribution: T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL) Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect Modifications: Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity) Synonyms: Zeta chain associated protein kinase, SYK related tyrosine kinase Structure: Protein tyrosine kinase, contains two SH2 domains, 70 kD

Application Details

Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis or Western blotting. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume. For Western blotting, the suggested working dilution(s)is 0.5-1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration 0.2 mg/ml.

Storage: The antibody solution should be stored undiluted at 4°C.

Research Area: Signaling, Tags/Labels, Immunology, Cancer

Restrictions: For Research Use only

Images

Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Publications

Publications: Chan, Iwashima, Turck et al.: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992 (PubMed).

Arpaia, Shahar, Dadi et al.: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994 (PubMed).

Chan, Kadlecek, Elder et al.: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

Qian, Lev, van Oers et al.: "Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling." in: The Journal of experimental medicine, Vol. 185, Issue 7, pp. 1253-9, 1997 (PubMed).

Crespo, Bosch, Villamor et al.: "ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia." in: The New England journal of medicine, Vol. 348, Issue 18, pp. 1764-75, 2003 (PubMed).