ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
At least three isoforms of SPATA3 are known to exist, this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members
Purification
Affinity chromatography purified via peptide column
Immunogen
SPATA3 antibody was raised against a AA 17 synthetic peptide near the carboxy terminus of human SPATA3.
SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Optimal dilutions for each application to be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage
4 °C/-20 °C
Storage Comment
SPATA3 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.
Synonyms: Spermatogenesis associated protein 3, testis and spermatogenesis cell apoptosis related gene 1, TSARG1
The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.