KDM5D antibody

Catalog No. ABIN1589829

Product Details anti-KDM5D Antibody

Target: KDM5D (Lysine (K)-Specific Demethylase 5D (KDM5D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM5D antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recombinant human SMCY

Characteristics: Chromosomal location: Yq11, Yq11

Isotype: IgG

Application Details

Application Notes: Western Blot: use 1-5 µg/mL

Comment:

Rabbit IG Polyclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for KDM5D

Target: KDM5D (Lysine (K)-Specific Demethylase 5D (KDM5D))

Alternative Name: SMCY/JARID1D (KDM5D Products)

Synonyms: JARID1D antibody, Jarid1d antibody, DKFZp468I1010 antibody, HY antibody, HYA antibody, SMCY antibody, Smcy antibody, lysine demethylase 5D antibody, lysine (K)-specific demethylase 5D antibody, lysine demethylase 5C antibody, KDM5D antibody, Kdm5d antibody, KDM5C antibody

Background: H-Y antigen is defined as a male histocompatibility antigen that causes rejection of male skin grafts by female recipients of the same inbred strain of rodents. Male-specific, or H-Y antigen(s), are also detected by cytotoxic T cells and antibodies. H-Y antigen appears to be an integral part of the membrane of most male cells. In addition, H-Y antibodies detect a soluble form of H-Y that is secreted by the testis. The gene (Smcy/SMCY) coding for H-Y antigen detected by T cells has been cloned. It is expressed ubiquitously in male mice and humans, and encodes an epitope that triggers a specific T-cell response in vitro. Additional epitopes coded for by different Y-chromosomal genes are probably required in vivo for the rejection of male grafts by female hosts. The molecular nature of H-Y antigen detected by antibodies on most male cells is not yet known. Testis-secreted, soluble H-Y antigen, however, was found to be identical to Müllerian-inhibiting substance (MIS). MIS cross-reacts with H-Y antibodies and identical findings were obtained for soluble H-Y antigen and MIS, i.e., secretion by testicular Sertoli and, to a lesser degree, ovarian cells, binding to a gonad-specific receptor, induction of gonadal sex reversal in vitro and, in cattle, in vivo. H-Y antisera also detect a molecule or molecules associated with the heterogametic sex in non-mammalian vertebrates. Molecular data on this antigen or antigens are not yet available.
Synonyms: KDM5D lysine (K)-specific demethylase 5D, HY, HYA

NCBI Accession: NM_001146705, NP_001140177

UniProt: Q9BY66

Pathways: Warburg Effect