IL-2 antibody

Antigen: IL-2

Clonality: Monoclonal (MQ1-17H12)

Host: Rat

Reactivity: Human, Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey

Application: ELISA (Capture), Intracellular Flow Cytometry (ICFC), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN160070

Additional Information

Immunogen: E. coli - expressed recombinant human IL-2

Format: Purified

Isotype: IgG2a, kappa chain  (Matching secondary antibodies)

Clone: MQ1-17H12

Description: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance T cells IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The MQ1-17H12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Synonyms: Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF) Regulation: Upregulated by NFAT, downregulated by TCF-8, CIF (colostrums inhibitory factor) Structure: Cytokine, 15.4 kD (Mammalian)

Application Details

Application Notes: ELISA or ELISPOT Capture: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5111 antibody (ABIN160627) as the detecting antibody. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MQ1-17H12 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of human IL-2 bioactivity (ABIN160081). Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-2 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4° C.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer

Restrictions: For Research Use only

Images

anti-IL-2 antibody

Publications

Publications: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).

Fernandez, Andersson, Andersson et al.: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, 1994 (PubMed).

Nistico: "Communications among central nervous system, neuroendocrine and immune systems: interleukin-2." in: Progress in neurobiology, Vol. 40, Issue 4, pp. 463-75, 1993 (PubMed).

Skansuen-Saphir, Andersson, Bjoerk et al.: "Lymphokine production induced by streptococcal pyrogenic exotoxin-A is selectively down-regulated by pooled human IgG." in: European journal of immunology, Vol. 24, Issue 4, pp. 916-22, 1994 (PubMed).

Waldmann, Goldman, Top et al.: "The interleukin-2 receptor: a target for immunotherapy." in: Annals of the New York Academy of Sciences, Vol. 685, pp. 603-10, 1993 (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Raqib, Ekberg, Sharkar et al.: "Apoptosis in acute shigellosis is associated with increased production of Fas/Fas ligand, perforin, caspase-1, and caspase-3 but reduced production of Bcl-2 and interleukin-2." in: Infection and immunity, Vol. 70, Issue 6, pp. 3199-207, 2002 (PubMed).