IL-2 antibody (FITC)

Antigen: IL-2

Clonality: Monoclonal (MQ1-17H12)

Host: Rat

Reactivity: Human, Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey

Conjugate: FITC

Application: Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN160073

Additional Information

Immunogen: E. coli - expressed recombinant human IL-2

Isotype: IgG2a, kappa chain

Clone: MQ1-17H12

Description: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance T cells IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The MQ1-17H12 antibody can neutralize the bioactivity of natural or recombinant IL-2. Synonyms: Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF) Regulation: Upregulated by NFAT, downregulated by TCF-8, CIF (colostrums inhibitory factor) Structure: Cytokine, 15.4 kD (Mammalian)

Application Details

Application Notes: ELISA or ELISPOT Capture: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5111 antibody (ABIN160627) as the detecting antibody. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MQ1-17H12 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of human IL-2 bioactivity (ABIN160081). Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. µg format: For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume test format: For immunofluorescent staining, the suggested use of this reagent is 20 µl per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Buffer: µg format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer

Restrictions: For Research Use only

Images

PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cy5 and MQ1-17H12 PE

Publications

Publications: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).

Fernandez, Andersson, Andersson et al.: "Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid." in: European journal of immunology, Vol. 24, Issue 8, pp. 1808-15, 1994 (PubMed).

Nistico: "Communications among central nervous system, neuroendocrine and immune systems: interleukin-2." in: Progress in neurobiology, Vol. 40, Issue 4, pp. 463-75, 1993 (PubMed).

Skansuen-Saphir, Andersson, Bjoerk et al.: "Lymphokine production induced by streptococcal pyrogenic exotoxin-A is selectively down-regulated by pooled human IgG." in: European journal of immunology, Vol. 24, Issue 4, pp. 916-22, 1994 (PubMed).

Waldmann, Goldman, Top et al.: "The interleukin-2 receptor: a target for immunotherapy." in: Annals of the New York Academy of Sciences, Vol. 685, pp. 603-10, 1993 (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Raqib, Ekberg, Sharkar et al.: "Apoptosis in acute shigellosis is associated with increased production of Fas/Fas ligand, perforin, caspase-1, and caspase-3 but reduced production of Bcl-2 and interleukin-2." in: Infection and immunity, Vol. 70, Issue 6, pp. 3199-207, 2002 (PubMed).