GM-CSF antibody (PE)

Antigen: GM-CSF

Synonyms: GMCSF, GM-CSF

Clonality: Monoclonal (MP1-22E9)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Intracellular Flow Cytometry (ICFC)

Catalog no.: ABIN160405

Additional Information

Immunogen: Yeast-expressed, recombinant mouse GM-CSF.

Isotype: IgG2a, kappa chain

Clone: MP1-22E9

Description: Growth/development granulocyte/macrophage progenitors, differentiates myeloblasts/monoblasts, synergizes with Epo proliferation of erythroid/megakaryocytic progenitors T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells. The MP1-22E9 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF). The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. Synonyms: Granulocyte/macrophage-colony stimulating factor, CSF-alpha, Pluripoietin-alpha, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA) Regulation: Synergistic with IL-1, IL-3, G-CSF, E21R competitive antagonist for receptor binding, stored in ECM with heparan sulfate proteoglycans Structure: Cytokine, 22 kD (Mammalian)

Application Details

Application Notes: ELISA or ELISPOT Capture: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (ABIN160410) as the detecting antibody. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse GM-CSF bioactivity in vivo and in vitro (ABIN160408). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Restrictions: For Research Use only

Publications

Publications: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Negrin, Greenberg: "Therapy of hematopoietic disorders with recombinant colony-stimulating factors." in: Advances in pharmacology (San Diego, Calif.), Vol. 23, pp. 263-96, 1992 (PubMed).

Demetri, Griffin: "Granulocyte colony-stimulating factor and its receptor." in: Blood, Vol. 78, Issue 11, pp. 2791-808, 1991 (PubMed).

Fan, OBrian, Ioannides et al.: "Granulocyte-macrophage colony-stimulating factor (GM-CSF) in the management of cancer." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 571-7, 1992 (PubMed).

Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, 1991 (PubMed).

Nozaki, Abrams, Pearce et al.: "Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes." in: The Journal of investigative dermatology, Vol. 97, Issue 1, pp. 10-4, 1991 (PubMed).

Suda, OGarra, MacNeil et al.: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, 1990 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Larkin, Renukaradhya, Sriram et al.: "CD44 differentially activates mouse NK T cells and conventional T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 268-79, 2006 (PubMed).