G-CSF antibody (Biotin)

Antigen: G-CSF

Clonality: Monoclonal (BVD11-37G10)

Host: Rat

Reactivity: Human

Conjugate: Biotin

Application: ELISA (Detection), ELISPOT (Detection)

Catalog no.: ABIN160485

Additional Information

Immunogen: E. coli-expressed, recombinant human G-CSF

Isotype: IgG2a, kappa chain

Clone: BVD11-37G10

Description: Growth/differentiation/activating factor for neutrophils, proliferation/migration endothelial cells cells, synergistic with IL-3 growth of hematopoietic progenitors Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma. The BVD11-37G10 antibody reacts with human granulocyte-colony stimulating factor (G-CSF).The BVD11-37G10 antibody can neutralize the bioactivity of natural or recombinant G-CSF. Synonyms: Granulocyte-Colony Stimulating Factor, CSF-beta, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2) Regulation: Upregulated by IL-1, IL-17, TNF-alpha, IFN-gamma, GM-CSF, downregulated by prostaglandin E2 Structure: Cytokine, 21 kD (Mammalian)

Application Details

Application Notes: ELISA or ELISPOT Detection: The biotinylated BVD11-37G10 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD13-3A5 antibody (ABIN160203/ABIN160205) as the capture antibody. Additional reported applications (for the relevant formats) include: Western blotting, neutralization, and immunohistochemistry. Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Purification: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Restrictions: For Research Use only

Images

anti-G-CSF antibody (Biotin)

Publications

Publications: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Negrin, Greenberg: "Therapy of hematopoietic disorders with recombinant colony-stimulating factors." in: Advances in pharmacology (San Diego, Calif.), Vol. 23, pp. 263-96, 1992 (PubMed).

Demetri, Griffin: "Granulocyte colony-stimulating factor and its receptor." in: Blood, Vol. 78, Issue 11, pp. 2791-808, 1991 (PubMed).

Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, 1991 (PubMed).

Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).

Schulz, Reeves, Hoad et al.: "Effect of mutations in the V3 loop of HIV-1 gp120 on infectivity and susceptibility to proteolytic cleavage." in: AIDS research and human retroviruses, Vol. 9, Issue 2, pp. 159-66, 1993 (PubMed).