Aldolase antibody (Biotin)

Catalog No. ABIN1607664

Product Details anti-Aldolase Antibody (Biotin)

Target: Aldolase (ALD)

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Aldolase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Aldolase from other sources may occur but have not been specifically determined.

Purity: Anti-Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].

Endotoxin Level: Low Endotoxin : No

Immunogen: Aldolase [Rabbit Muscle]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Aldolase has been assayed against 1.0 µg of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:16.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Aldolase

Target: Aldolase (ALD)

Alternative Name: Aldolase (ALD Products)

Synonyms: ALD antibody, BcDNA:LP07735 antibody, CG6058 antibody, Dmel\\CG6058 antibody, FBA antibody, T12 antibody, T4 antibody, T6 antibody, T7 antibody, ald antibody, Aldolase antibody, Ald antibody

Background: Part of the class I fructose-bisphosphate aldolase family, the Anti-Aldolase antibody is essential in the processes glycolysis and gluconeogenesis, as well as performing the role of a scaffolding protein. Anti-Aldolase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Fructose-bisphosphate aldolase A Muscle-type aldolase

Gene ID: 100009055

UniProt: P00883