AK3 antibody
| Antigen |
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| Clonality |
Monoclonal (SJB3-36) |
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN165377 |
| Quantity |
50ul (1mg/ml) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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Recombinant human AK3 (adenylaste kinase isozyme 3) purified from E. coli. Anti-human AK3 mAb, clone SJB3-36, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AK3 protein.
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Isotype
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IgG1 kappa (Matching secondary antibodies)
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Clone
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SJB3-36
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Description
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Adenylate kinase (AK, adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.
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Application Notes
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The antibody has been tested by ELISA , Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2,000. This antibody(SJB3-36) has the specificity against AK3. Recommended starting dilution is 1:1,000.
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Concentration
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1mg/ml
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Purification
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The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Can be stored at 4C for up to one month, but store at -20C for long term storage. Avoid repeated freezing and thawing cycles.
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Buffer
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Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
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Restrictions
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For Research Use only
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Alternatives for antigen "AK3", type "Antibodies"
