Anthranilic Acid antibody

Antigen: Anthranilic Acid

Clonality: Polyclonal

Host: Rabbit

Catalog no.: ABIN165520

Additional Information

Immunogen: Synthetic Anthranilic acid conjugated to protein carriers.

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Specificity: Conjugated Anthranilic acid . Using a conjugate Anthranilic acid-protein carrier, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a) Anthranilic acid-BSA 1 3OH Anthranilic acid-BSA 1/35 Kynurenic acid-BSA 1/8,500 Quinaldic acid-BSA 1/>10,000 Picolinic acid-BSA 1/>50,000 3OH Kynurenine-BSA 1/>10,000 Kynurenine 1/>50,000 Xanthurenic acid-BSA 1/>50,000 (a)

Application Details

Application Notes: Example of Immunohistochemistry protocol Perfusion protocol for Adult male Sprague Dawley (weight around 0.5 kg):1-The animals can be deeply anaesthetized (for example with urethane-0.5-1.5g/kg, intraperitoneal). 2-Heparinized, and perfused via the ascending aorta with 50 ml of MES (2-Morpholinoethanesulfonic acid monohydrate Fluka) 10 -1 Immunohistochemical protocol:1-In order to avoid possible interference with endogenous peroxidase, free-floating sections will be treated with distilled water containing NH 3 (20%), H 2 O 2 (30%) and NaOH (1%) for 20 min (other method is using a solution with 33% of H 2 O 2 and 66% of methanol). 2-Then, wash the sections for 20 min in 0.15 M phosphate-buffered saline (PBS) (pH 7.2) 3-Pre-incubate for 30 min in PBS containing 10% of normal horse serum and 0.3% of Triton X-100 (mixed solution). 4-Incubate at room temperature (1h 30min) and overnight at 4º C in the same mixed solution containing anti-conjugated Anthranilic acid antibodies (diluted 1/1,000-1,5000 as recommended dilution). 5-Then, the sections will be wash in PBS (30 min). 6-After that we will incubate for 60 min at room temperature with biotinylated anti-rabbit immunogammaglobulin (Vector) diluted 1/200 in PBS. 7-Wash during 30 min with PBS. 8-Sections will be incubated for 1 h with a 1/100 diluted avidin-biotin-peroxidase complex (Vectastain). 9-After that we will wash the sections in PBS (30 min) 10-Wash with Tris-HCl buffer (pH 7.6)(10 min). 11-The tissue-bound peroxidase will be developed with H 2 O 2 using 3, 3' diaminobenzidine as chromogen. 12-Finally the sections will be rinsed with PBS and coverslipped with PBS/Glycerol (1/1). Recommended dilutions for Immunochemistry (1/1,000-1/5,000). Recommended dilutions for Western blot (1/1,000-1/2,000).

Purity: Antiserum previously absorbed on protein carriers, and purified.

Storage: After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times), and stable at least 2 years.

Restrictions: For Research Use only