Cystathionase (Cystathionine gamma-Lyase) (CTH) antibody

Antigen: Cystathionase (Cystathionine gamma-Lyase) (CTH)

Synonyms: CTH, DKFZp469D0332, zgc:66001, zgc:85785, wu:fb48e03, wu:fb58d08, Cys3, AI314617, BC019483, MGC28655, 0610010I13Rik, MGC9471, cysA, SCQ11.03c, H, MGC75946

Clonality: Monoclonal (S51)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN166006

Additional Information

Gene ID: 1491

Immunogen: CTH (AAH15807, 1 a.a. ~ 406 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence: (without GST) MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQH SGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICM DDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQK VIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGHSDV VMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMA VAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLK NLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEED LLEDLDQALKAAHPPSGSHS*

Isotype: IgG1-kappa  (Matching secondary antibodies)

Clone: S51

Description: Cystathionase (cystathionine gamma-lyase) This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. Mouse monoclonal antibody raised against a full length recombinant CTH. Synonyms: MGC9471

Application Details

Application Notes: Quality Control: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Buffer: In 1x PBS, pH 7.2 Western Blot detection against Immunogen (70.66 KDa) .

Storage: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

Western Blot detection against Immunogen (70.66 KDa) .

Immunoperoxidase of monoclonal antibody to CTH (H00001491-M03) on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

Publications

Publications: Kandil, Brennan, McBean: "Glutathione depletion causes a JNK and p38MAPK-mediated increase in expression of cystathionine-gamma-lyase and upregulation of the transsulfuration pathway in C6 glioma cells." in: Neurochemistry international, Vol. 56, Issue 4, pp. 611-9, 2010 (PubMed).