IL-10 antibody
-
- Target See all IL-10 (IL10) Antibodies
- IL-10 (IL10) (Interleukin 10 (IL10))
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This IL-10 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purification
- Purified
- Sterility
- Sterile filtered
- Immunogen
- Polyclonal Interleukin-10 antibody was produced from sera of rabbits immunized with synthetic protein containing Interleukin-10 antigenic determinants.
- Isotype
- IgG
- Top Product
- Discover our top product IL10 Primary Antibody
-
-
- Application Notes
-
ELISA: Use at a concentration range of 1-2 µg/mL.
Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL.
IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
- Buffer
- PBS solution at a concentration of 1 mg/mL.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Recombinant CD80, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.
- Expiry Date
- 12 months
-
- Target
- IL-10 (IL10) (Interleukin 10 (IL10))
- Alternative Name
- IL-10 (IL10 Products)
- Synonyms
- CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody
- Background
- CD80 is a monomeric surface protein found on activated APCs. The extracellular region of CD80 consists of two highly glycosylated Ig-like extracellular domains, which have defined regions for binding its ligands, CD28 or CTLA-4, on T cells. One ligand of CD80, CD28, provides critical costimulatory signals to activated T cells to increase cytokine production, cell proliferation, and cell viability, while the other ligand, CTLA-4, may mediate a growth-inhibitory signal. The same regions of CD80 are involved in binding to both CD28 and CTLA-4, but CTLA-4 binds to CD80 with about a0-fold higher avidity than CD28 does. This suggests that low levels of CTLA-4 expressed on the cell surface can compete for CD80 binding even when CD28 is expressed at much higher levels.
- UniProt
- P22301
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
-