NQO1 antibody (NAD(P)H Dehydrogenase, Quinone 1) (C-Term)

Details for Product anti-NQO1 Antibody No. ABIN1686087, Supplier: Log in to see
Antigen
  • zgc:77191
  • wu:fb63c10
  • nqo1
  • DHQU
  • DIA4
  • DTD
  • NMOR1
  • NMORI
  • QR1
  • Dia4
  • AV001255
  • Dtd
  • Nmo-1
  • Nmo1
  • Nmor1
  • Ox-1
  • Ox1
  • Qr1
  • NADPH-d
  • NAD(P)H dehydrogenase, quinone 1
  • nqo1
  • NQO1
  • Bpet2092
  • Nqo1
Epitope
C-Term
32
15
11
9
7
6
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
223
77
67
39
20
14
7
6
4
3
1
1
Host
Rabbit
94
91
36
2
Clonality
Polyclonal
Conjugate
This NQO1 antibody is un-conjugated
12
6
6
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
198
71
64
57
44
38
37
27
17
13
5
3
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Polyclonal Antibody to detect NQO1 in human, mouse and rat samples
Immunogen Synthetic peptide at C-terminal
Blocking Peptide Blocking peptide for this product available: ABIN1672714
Isotype IgG
Characteristics Polyclonal Antibody to detect NQO1 in human, mouse and rat samples
Purification Affinity purified
Alternative Name NQO1 (NQO1 Antibody Abstract)
Background NAD (P) H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
Synonyms: Azoreductase, DT-diaphorase, Menadione reductase, NAD (P) H, quinine oxidoreductase 1
Gene ID 1728
UniProt P15559
Research Area Signaling, Metabolism
Application Notes Western blot: 1:200
Comment

Suggested positive control:
Western blot: 3T3 cell lysate, rat kidney cell lysate

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (C-Term) antibody (ABIN1686087) anti-NAD(P)H Dehydrogenase, Quinone 1 (NQO1) (C-Term) antibody
Product cited in: La Sala, Pujadas, De Nigris, Canivell, Novials, Genovese, Ceriello: "Oscillating glucose and constant high glucose induce endoglin expression in endothelial cells: the role of oxidative stress." in: Acta diabetologica, 2014 (PubMed).

Did you look for something else?