KRT18 antibody (Keratin 18) (C-Term)

Details for Product anti-KRT18 Antibody No. ABIN1688258, Supplier: Log in to see
Antigen
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • CYK18
  • K18
  • CK18
  • Krt1-18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krtt1c6
  • zgc:66015
  • keratin 18
  • similar to Keratin, type I cytoskeletal 18 (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
  • Keratin, type I cytoskeletal 18
  • cytokeratin 18
  • K18, simple type I keratin
  • zgc:77517
  • krt18
  • KRT18
  • k1c18
  • LOC100008885
  • LOC100136778
  • Krt18
  • zgc:77517
Alternatives
anti-Human KRT18 antibody for Immunohistochemistry (Frozen Sections)
Epitope
C-Term
51
36
27
23
20
16
15
11
11
10
9
8
7
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
947
200
171
71
65
41
36
34
27
27
23
20
18
15
12
7
7
5
5
4
3
3
2
2
1
1
1
Host
Rabbit
699
274
12
11
4
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This KRT18 antibody is un-conjugated
52
48
22
18
17
17
14
14
14
14
14
14
14
14
14
11
11
8
4
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
656
466
389
349
331
300
225
180
125
40
26
18
18
14
11
9
3
2
2
1
1
1
Options
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Supplier Product No.
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Immunogen Synthetic peptide corresponding to C-terminus of human Keratin 18.
Clone SP69
Isotype IgG
Predicted Reactivity Cow (Bovine),Frog,Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Cytoplasm
Purification Purified by Protein A/G
Alternative Name Keratin 18 (KRT18 Antibody Abstract)
Background Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with cytokeratin 8, a basic keratin. Hepatocellular carcinomas express only cytokeratins 8 and 18.
Molecular Weight 52 kDa
Gene ID 3875
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes IHC Dilution: Ready to use, for IHC only
Comment

Positive Control:
IHC (p): Prostate, breast adenocarcinoma
WB: A431 cell lysate
FC: HeLa Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only
Format Liquid
Buffer pre-diluted rabbit monoclonal antibody purified by protein A/G in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide. (For IHC only).
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C
Supplier Images
Flow Cytometry (FACS) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) Flow cytometric analysis of rabbit anti-Keratin 18 (SP69) antibody in HeLa (green) co...
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 11)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 12)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 13)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 14)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 15)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 16)
Western Blotting (WB) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1688258) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 17)
Product cited in: Krasteva-Christ, Soultanova, Schütz, Papadakis, Weiss, Deckmann, Chubanov, Gudermann, Voigt, Meyerhof, Boehm, Weihe, Kummer: "Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts." in: International immunopharmacology, Vol. 29, Issue 1, pp. 158-65, 2015 (PubMed).