Atic/Aicar Transformylase (AA 351-450) antibody (HRP)

Catalog No. ABIN1712181

Product Details

Target: Atic/Aicar Transformylase

Binding Specificity: AA 351-450

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATIC/AICAR transformylase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Atic/Aicar Transformylase

Background:

Synonyms: 5 aminoimidazole 4 carboxamide 1 beta D ribonucleotide transformylase/inosinicase, 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase, 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase, AICAR, AICAR formyltransferase/IMP cyclohydrolase bunctional enzyme, AICAR transformylase, AICARFT, AICARFT/IMPCHASE, ATIC, Bunctional purine biosynthesis protein PURH, FLJ93545, IMP cyclohydrolase, IMP synthase, IMP synthetase, IMPCHASE, Inosinicase, OK/SW-cl.86, Phosphoribosylaminoimidazolecarboxamide formyltransferase, Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, PUR9_HUMAN, PURH.

Background: The bifunctional purine biosynthesis protein ATIC (also designated PURH) contains AICAR transformylase and IMP cyclohydrolase activities. AICAR (5-aminoimidazole-4-carboxamide ribonucleotide) transformylase catalyzes the second to last step in purine biosynthesis, playing an important role in the production of nucleotides and IMP. Defects in the ATIC transformylase gene can cause AICA-rebsuria, also designated AICA-ribosiduria, an inborn error in purine biosynthesis that is neurologically cataclysmic. Individuals with AICA-rebosuria accumulate AICA-riboside, also designated ZMP, and its derivatives in erythrocytes and fibroblasts. Patients also excrete very large amounts of AICA-riboside in the urine. Mental retardation, epilepsy, dysmorphic features and congenital blindness are all symptoms of this disease.

Gene ID: 471