BLM antibody (AA 1201-1417) (HRP)

Catalog No. ABIN1712183

Product Details anti-BLM Antibody (HRP)

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Binding Specificity: AA 1201-1417

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BLM/Blooms Syndrome Protein Blm

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM/Blooms Syndrome Protein Blm (BLM Products)

Synonyms: BS antibody, RECQ2 antibody, RECQL2 antibody, RECQL3 antibody, blm-A antibody, xBLM antibody, ORF1 antibody, Bloom syndrome RecQ like helicase antibody, Bloom syndrome RecQ like helicase S homeolog antibody, Bloom syndrome, RecQ like helicase antibody, BLM antibody, blm.S antibody, Blm antibody

Background:

Synonyms: BLM, BLM_HUMAN, Bloom Syndrome, Bloom syndrome protein, Bloom syndrome RecQ helicase like, BS, DNA Helicase, DNA helicase RecQ like type 2, MGC126616, MGC131618, MGC131620, RECQ 2, RECQ like, RecQ like type 2, RecQ protein like 3, RecQ Protein-like 3, RECQ-2, RECQ-Like, RecQ-like type 2, RECQ2, RECQL 2, RECQL 3, RECQL-2, RECQL-3, RECQL2, RECQL3, type 2.

Background: Bloom?s syndrome is an autosomal recessive disorder characterized by pre- and post-natal growth deficiencies, sun sensitivity, immunodeficiency and a predisposition to various cancers. The gene responsible for Bloom?s syndrome, BLM, encodes a protein homologous to the RecQ helicase of E. coli and is mutated in most Bloom?s syndrome patients. One characteristic of Bloom?s syndrome is an increased frequency of sister chromatid exchange (SCE). BLM has been shown to unwind G4 DNA, and a failure of this function is thought to be responsible for the increased rate of SCE. BLM is known to be translocated to the nucleus, where its ATPase activity is stimulated by both single- and double-stranded DNA. Mutations in the yeast SGS1, a homolog of BLM, are known to cause mitotic hyperrecombination similiar to that observed in Bloom?s cells.

Gene ID: 641

Pathways: DNA Damage Repair