Aconitase 1 antibody (pSer138) (HRP)

Catalog No. ABIN1712476

Product Details anti-Aconitase 1 Antibody (HRP)

Target: Aconitase 1 (ACO1)

Binding Specificity: pSer138

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Aconitase 1 around the phosphorylation site of Ser138 [AD(p-S)LQ]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: Aconitase 1 (ACO1 Products)

Synonyms: ACONS antibody, IREB1 antibody, IREBP antibody, IREBP1 antibody, IRP1 antibody, Acon1 antibody, Ratireb antibody, ratireb antibody, Aco1 antibody, AI256519 antibody, Aco-1 antibody, Irebp antibody, Irp1 antibody, aconitase 1 antibody, aconitase 1, soluble antibody, aconitase 1 L homeolog antibody, ACO1 antibody, Aco1 antibody, aco1.L antibody

Background:

Synonyms: Aconitase 1 phospho S138, p-Aconitase 1 phospho S138, ACO 1, ACO1, ACOC_HUMAN, Aconitase 1 soluble, Aconitase, Aconitase1, Aconitate hydratase, ACONS, Citrate hydro lyase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRE BP 1, IRE-BP 1, IREB 1, IREB1, IREBP, IREBP1, Iron regulatory protein 1, Iron responsive element binding protein 1, Iron-responsive element-binding protein 1, IRP 1, IRP1, OTTHUMP00000045233.

Background: Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins, iron regulatory protein-1 and -2 (IRP-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IREBP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide.

Gene ID: 48

Pathways: Transition Metal Ion Homeostasis