PRKCSH antibody (AA 101-200) (HRP)

Catalog No. ABIN1712538

Product Details anti-PRKCSH Antibody (HRP)

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Binding Specificity: AA 101-200

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKCSH antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glucosidase 2 subunit beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Alternative Name: Glucosidase 2 subunit beta (PRKCSH Products)

Synonyms: AGE-R2 antibody, G19P1 antibody, PCLD antibody, PKCSH antibody, PLD1 antibody, 80K-H antibody, VASAP-60 antibody, zgc:55653 antibody, prkcsh antibody, MGC75615 antibody, MGC82696 antibody, PRKCSH antibody, protein kinase C substrate 80K-H antibody, protein kinase C substrate 80K-H L homeolog antibody, phospholipase D1 antibody, PRKCSH antibody, Prkcsh antibody, prkcsh antibody, prkcsh.L antibody, Bm1_42055 antibody, LOC100304710 antibody, LOC100526729 antibody, PLD1 antibody

Background:

Synonyms: 80K-H protein, AGE-binding receptor 2, AGE-R2, G19P1, GLU2B_HUMAN, Glucosidase 2 subunit beta, Glucosidase II beta subunit, Glucosidase II subunit beta, Hepatocystin, PCLD, PKCSH, PLD1, PRKCSH, Protein kinase C substrate 60.1 kDa protein heavy chain, Protein kinase C substrate 80 Kda protein, Protein kinase C substrate 80K-H, Protein kinase C substrate, 80 Kda protein.

Background: Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains a and b subunits (Glucosidase IIa and Glucosidase IIb) which form a defined heterodimeric complex. Glucosidase IIa is the catalyitc core of the enzyme and can function independently of the b subunit. The sequence of Glucosidase IIb encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C-terminus. The phosphorylated form of Glucosidase IIb is localized in the plasma membrane and is highly expressed in FGF-stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIb was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIb retains the complete complex in the ER.

Gene ID: 5589

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process