GPR31 antibody (AA 121-220)

Catalog No. ABIN1713499

Product Details anti-GPR31 Antibody

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Binding Specificity: AA 121-220

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human G protein coupled receptor 31

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GPR31

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Alternative Name: GPR31 (GPR31 Products)

Synonyms: 12-HETER antibody, HETER antibody, HETER1 antibody, GPR31 antibody, GPR31c(t) antibody, Gpr31 antibody, Gpr31c antibody, G protein-coupled receptor 31 antibody, G protein-coupled receptor 31, D17Leh66b region antibody, GPR31 antibody, Gpr31 antibody, Gpr31b antibody

Background:

Synonyms: HETER, HETER1, 12-HETER, 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor, 12-(S)-HETE receptor, G-protein coupled receptor 31, GPR31

Background: High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.

Gene ID: 2853

UniProt: O00270