DPPA3 antibody (AA 101-159)

Catalog No. ABIN1713577

Product Details anti-DPPA3 Antibody

Target: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Binding Specificity: AA 101-159

Reactivity: Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPPA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Pig

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Stella/DPPA3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-DPPA3 antibody (ABIN1713577)

Wasielak, Więsak, Bogacka, Jalali, Bogacki: "Zygote arrest 1, nucleoplasmin 2, and developmentally associated protein 3 mRNA profiles throughout porcine embryo development in vitro." in: Theriogenology, Vol. 86, Issue 9, pp. 2254-2262, (2016) (PubMed).

Target Details for DPPA3

Target: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Alternative Name: Stella/DPPA3 (DPPA3 Products)

Synonyms: STELLA antibody, 2410075G02Rik antibody, PCG7 antibody, PGC7 antibody, Stella antibody, developmental pluripotency associated 3 antibody, developmental pluripotency-associated 3 antibody, DPPA3 antibody, Dppa3 antibody

Background:

Synonyms: STELLA, Developmental pluripotency-associated protein 3, Stella-related protein, DPPA3, STELLAR

Background: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

Gene ID: 359787

UniProt: Q6W0C5