GATA3 antibody (pSer115)

Catalog No. ABIN1714274

Product Details anti-GATA3 Antibody

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Binding Specificity: pSer115

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Zebrafish (Danio rerio)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GATA3 around the phosphorylation site of Ser115

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3 (GATA3 Products)

Synonyms: HDR antibody, HDRS antibody, Gata-3 antibody, jal antibody, cb550 antibody, gta3 antibody, GATA-3 antibody, XGATA3 antibody, xgata-3 antibody, hdr antibody, xGATA-3 antibody, GATA3 antibody, GATA transcription factor 3 antibody, T4L20.260 antibody, T4L20_260 antibody, gata3 antibody, ZH-DR antibody, zgc:103531 antibody, GATA binding protein 3 antibody, GATA binding protein 3 S homeolog antibody, GATA transcription factor 3 antibody, amidase antibody, putative amidase antibody, GATA-binding protein 3 antibody, hematopoietic death receptor antibody, GATA3 antibody, Gata3 antibody, gata3 antibody, gata3.S antibody, gatA3 antibody, hdr antibody

Background:

Synonyms: GATA3 phospho S115, p-GATA3 phospho S115, GATA3 phospho Ser115, p-GATA3 Ser115, GATA 3, GATA3, GATA-3, GATA binding factor 3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, Trans acting T cell specic transcription factor GATA 3.

Background: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Gene ID: 2625

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation