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GFI1 antibody (AA 301-400)

GFI1 Reactivity: Rat, Mouse, Xenopus laevis WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1714623
  • Target See all GFI1 Antibodies
    GFI1 (Growth Factor Independent 1 (GFI1))
    Binding Specificity
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 301-400
    Reactivity
    • 22
    • 15
    • 14
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    Rat, Mouse, Xenopus laevis
    Host
    • 16
    • 6
    Rabbit
    Clonality
    • 18
    • 4
    Polyclonal
    Conjugate
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GFI1 antibody is un-conjugated
    Application
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Mouse, Rat, Xenopus laevis
    Predicted Reactivity
    Human,Dog,Sheep,Pig,Horse,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human GFI1/ZNF163
    Isotype
    IgG
    Top Product
    Discover our top product GFI1 Primary Antibody
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    GFI1 (Growth Factor Independent 1 (GFI1))
    Alternative Name
    GFI1/ZNF163 (GFI1 Products)
    Synonyms
    AW495828 antibody, Gfi-1 antibody, Pal-1 antibody, Pal1 antibody, GFI1 antibody, GFI-1 antibody, GFI1A antibody, SCN2 antibody, ZNF163 antibody, growth factor independent 1 antibody, growth factor independent 1 transcriptional repressor antibody, Gfi1 antibody, GFI1 antibody
    Background

    Synonyms: GFI 1, GFI1, GFI-1, GFI1_HUMAN, Growth factor independence 1, growth factor independent 1, Growth factor independent protein 1, SCN2, Zinc finger protein 163, Zinc finger protein Gfi 1, Zinc finger protein Gfi-1, Zinc finger protein Gfi1, ZNF 163, ZNF163.

    Background: Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transcriptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the Socs 1 and 3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.3, respectively.

    Gene ID
    2672
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